TM-04-176-01
Based on 1 Customer Validation
TM-04-176-01 (PI5P4Kα-IN-1) is a selective PIP4K2C inhibitor (Kd 3.4 nM) and PI5P4K inhibitor (IC50: 1.96 and 9.4 μM for PI5P4Kα and PI5P4Kβ, respectively). TM-04-176-01 inhibits SARS-CoV-2 replication with low cytotoxicity. TM-04-176-01 can be used for research on SARS-CoV-2 infection.
For research use only. We do not sell to patients.
- Purity : 99.60%
- CAS No.: 2428737-31-7
- Formula: C20H17N3O2S
- Molecular Weight:363.43
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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PIP4K2C 3.4 nM (Kd) |
PI5P4Kα 1.96 μM (IC50) |
PI5P4Kβ 9.4 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Calu-3 | EC50 |
0.6 μM
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Antiviral activity against SARS-CoV-2 in human Calu-3 cells infected at MOI 0.05 or 1 and assessed by plaque assay.
Antiviral activity against SARS-CoV-2 in human Calu-3 cells infected at MOI 0.05 or 1 and assessed by plaque assay.
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40640184 |
| Calu-3 | CC50 |
20 μM
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Cytotoxicity against human Calu-3 cells assessed as cell viability by alamarBlue assay; CC50 >20 μM.
Cytotoxicity against human Calu-3 cells assessed as cell viability by alamarBlue assay; CC50 >20 μM.
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40640184 |
In Vitro
TM-04-176-01 is a high-affinity PIP4K2C binder (Kd 3.4 nM) and inhibits PIKfyve in vitro with IC50 268 nM[2].
In cell-based assays, TM-04-176-01 selectively engages PIP4K2C (IC50 36.7 nM) over PIKfyve (IC50 10,000 nM)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2428737-31-7
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Appearance Solid
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Molecular Weight 363.43
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Formula C20H17N3O2S
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Color Off-white to light yellow
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SMILES
O=S(NC1=CC=C(C2=CC(C3=C4C=CNC4=CC=C3)=CN=C2)C=C1)(C)=O
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Synonyms
PI5P4Kα-IN-1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 200 mg/mL (550.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (13.76 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (13.76 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (285 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7516 mL | 13.7578 mL | 27.5156 mL | 68.7890 mL |
| 5 mM | 0.5503 mL | 2.7516 mL | 5.5031 mL | 13.7578 mL | |
| 10 mM | 0.2752 mL | 1.3758 mL | 2.7516 mL | 6.8789 mL | |
| 15 mM | 0.1834 mL | 0.9172 mL | 1.8344 mL | 4.5859 mL | |
| 20 mM | 0.1376 mL | 0.6879 mL | 1.3758 mL | 3.4395 mL | |
| 25 mM | 0.1101 mL | 0.5503 mL | 1.1006 mL | 2.7516 mL | |
| 30 mM | 0.0917 mL | 0.4586 mL | 0.9172 mL | 2.2930 mL | |
| 40 mM | 0.0688 mL | 0.3439 mL | 0.6879 mL | 1.7197 mL | |
| 50 mM | 0.0550 mL | 0.2752 mL | 0.5503 mL | 1.3758 mL | |
| 60 mM | 0.0459 mL | 0.2293 mL | 0.4586 mL | 1.1465 mL | |
| 80 mM | 0.0344 mL | 0.1720 mL | 0.3439 mL | 0.8599 mL | |
| 100 mM | 0.0275 mL | 0.1376 mL | 0.2752 mL | 0.6879 mL |
TM-04-176-01 Related Classifications
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