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Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!2025-02-06
1 Result for "
FRET biosensor
" in MedChemExpress (MCE) Product Catalog:
Products (1)
1 Results for "FRET biosensor" in MCE Product Catalog:
Cat. No.: HY-D3427
CAS No.: 3100316-45-5
Target:
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T-sCPY-s500R-H is a NADPH-based FRET ratiometric biosensor probe. T-sCPY-s500R-H forms a 1:1 FRET pair upon binding to the eDHFR-HaloTag fusion protein through conjugation of the donor s500R (green fluorescence) and the acceptor sCPY (red fluorescence) with the HaloTag ligand and the eDHFR ligand (TMP), respectively; NADPH binding to eDHFR induces a conformational change that converts sCPY from a colorless spirolactam form to a red fluorescent form, enabling quantification of NADPH levels through the ratio of red/green fluorescence intensity (Ex = 488 or 490 nm; green channel Em = 500-580 nm; red channel Em = 600-700 nm). T-sCPY-s500R-H is applicable to research in fields such as cell metabolism studies, oxidative stress and NADPH homeostasis analysis, tumor metabolism, and NADPH-modulating drug screening .
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Protocols
Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!A suitable model is crucial in drug screening experiments. Organs can mimic the three-dimensional functional structure of internal organs, have similar spatial organization to corresponding organs, maintain some key characteristics, and reproduce some physiological functions. They are widely used for modeling and personalized drug screening physiological functions.2025-02-06
