Cat. No.: HY-P5360

TFLLRN-NH2

Name: TFLLRN-NH2
Brand: MedChemExpress
SKU: HY-P5360
Price: 85.00 USD
Availability: InStock
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TFLLRN-NH2 is a PAR-1 peptide-selective agonist. TFLLRN-NH2 induces AMPK phosphorylation and attenuates LPS-induced osteoclastogenesis, NFATc1 expression, NF-κB activation, and IκBα degradation. TFLLRN-NH2 stimulates MAPK phosphorylation in hepatocellular carcinoma cells. TFLLRN-NH2 induces concentration-dependent contraction of isolated human renal artery rings, stimulates IL-6 release from human T cells, and also evokes intracellular Ca²⁺ mobilization in primary human renal tubular epithelial cells. TFLLRN-NH2 can be used in research related to hepatocellular carcinoma and acute lung injury.

For research use only. We do not sell to patients.

Purity : 99.71%
CAS No.: 244095-36-1
Formula: C₃₅H₅₉N₁₁O₈
Molecular Weight:761.91

Storage:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

TFLLRN-NH2 Chemical Structure

CAS No.: 244095-36-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]^[3]

PAR1

AMPK

NF-κB

IL-6

All Protease Activated Receptor (PAR) Isoforms :

All AMPK Isoforms :

All NF-κB Isoforms :

All p38 MAPK Isoforms :

All Interleukin Related Isoforms :

In Vitro

Anti-cancerInnate ImmunityCytokines & ChemokinesOthers

Anti-cancer

TFLLRN-NH2 (100 μM) promotes Matrigel invasion of HEP-3B, PHC1, and PHC2 hepatocellular carcinoma cells, and this effect is inhibited by EGCG (HY-13653) in a concentration-dependent manner^[2].
TFLLRN-NH2 (100 μM; 20 min) activates p42/p44 MAPKinase phosphorylation in HEP-3B cells^[2].
TFLLRN-NH2 (100 μM) induces EGCG-sensitive p42/p44 MAPKinase activation in PHC1 and PHC2 primary HCC cultures^[2].

Innate Immunity

TFLLRN-NH2 (1 µg/mL; 0-15 min) attenuates LPS-induced IκBα degradation in RAW264.7 cells^[1].

Innate Immunity

Cytokines & Chemokines

TFLLRN-NH2 attenuates LPS-induced osteoclastogenesis, NFATc1 expression, AMPK phosphorylation, and NF-κB transcription in RAW264.7 cells^[1].

Others

TFLLRN-NH2 stimulates the release of IL-6 from purified human peripheral blood T cells^[3].
TFLLRN-NH2 induces a significant, concentration-dependent contractile effect in isolated human renal artery rings^[4].
TFLLRN-NH2 activates PAR-1 and induces intracellular Ca²⁺ mobilization in primary human tubular epithelial cells (HTECs); this peptide does not cross-activate PAR-2, cannot mediate PAR-1 transactivation of PAR-2, and fails to induce tissue factor (TF) synthesis in HTECs^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

RAW264.7 mouse monocyte/macrophage lineage cells
Concentration:

1 µg/mL (with LPS 1 µg/mL)
Incubation Time:

0, 5, 15 min
Result:

Attenuated LPS-induced IκBα degradation in RAW264.7 cells.

Western Blot Analysis^[2]

Cell Line:

human HCC cell line HEP-3B
Concentration:

100 μM
Incubation Time:

20 min
Result:

Caused an increase in phospho-p42/p44 MAPKinases in HEP-3B cells at 100 μM after 20 min.

Related Protocols :

Chemical Information

CAS No. 244095-36-1
Appearance Solid
Molecular Weight 761.91
Formula C₃₅H₅₉N₁₁O₈
Color White to off-white
Sequence

Thr-Phe-Leu-Leu-Arg-Asn-NH2
Sequence Shortening

TFLLRN-NH2
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (65.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3125 mL

6.5625 mL

13.1249 mL

5 mM

0.2625 mL

1.3125 mL

2.6250 mL

10 mM

0.1312 mL

0.6562 mL

1.3125 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kanno Y, et al. uPA Attenuated LPS-induced Inflammatory Osteoclastogenesis through the Plasmin/PAR-1/Ca(2+)/CaMKK/AMPK Axis. International journal of biological sciences. 2016;12(1):63-71.

[2]. Kaufmann R, et al. Green tea polyphenol epigallocatechin-3-gallate inhibits thrombin-induced hepatocellular carcinoma cell invasion and p42/p44-MAPKinase activation. Oncology reports. 2009 May;21(5):1261-7.

[3]. Li T, et al. Induction of IL‐6 release from human T cells by PAR‐1 and PAR‐2 agonists. Immunology and cell biology. 2006 Oct;84(5):461-6.

[4]. Tognetto M, et al. Proteinase-activated receptor-1 (PAR-1) activation contracts the isolated human renal artery in vitro. British journal of pharmacology. 2003 May;139(1):21-7.

[5]. Iyer A, et al. PAR2 Activation on Human Kidney Tubular Epithelial Cells Induces Tissue Factor Synthesis, That Enhances Blood Clotting. Frontiers in physiology. 2021;12:615428.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3125 mL	6.5625 mL	13.1249 mL	32.8123 mL
	5 mM	0.2625 mL	1.3125 mL	2.6250 mL	6.5625 mL
	10 mM	0.1312 mL	0.6562 mL	1.3125 mL	3.2812 mL
	15 mM	0.0875 mL	0.4375 mL	0.8750 mL	2.1875 mL
	20 mM	0.0656 mL	0.3281 mL	0.6562 mL	1.6406 mL
	25 mM	0.0525 mL	0.2625 mL	0.5250 mL	1.3125 mL
	30 mM	0.0437 mL	0.2187 mL	0.4375 mL	1.0937 mL
	40 mM	0.0328 mL	0.1641 mL	0.3281 mL	0.8203 mL
	50 mM	0.0262 mL	0.1312 mL	0.2625 mL	0.6562 mL
	60 mM	0.0219 mL	0.1094 mL	0.2187 mL	0.5469 mL

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TFLLRN-NH2

All Protease Activated Receptor (PAR) Isoforms

All AMPK Isoforms

All NF-κB Isoforms

All p38 MAPK Isoforms

All Interleukin Related Isoforms