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Cat. No.: HY-155667

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Z-Nle-Lys-Arg-AMC

Z-Nle-Lys-Arg-AMC is a selective Fluorescent peptide substrate for Cathepsin B. Z-Nle-Lys-Arg-AMC releases AMC upon cleavage, thereby enabling monitoring of proteolytic activity over an acidic to neutral pH range.

For research use only. We do not sell to patients.

CAS No.: 1135364-14-5
Formula: C₃₆H₅₀N₈O₇
Molecular Weight:706.83

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

Z-Nle-Lys-Arg-AMC Chemical Structure

CAS No.: 1135364-14-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

Cathepsin B

All Cathepsin Isoforms :

In Vitro

Z-Nle-Lys-Arg-AMC (40 μM; 10 min) is a highly efficient fluorogenic substrate for recombinant h-Cathepsin B, with k_cat/K_m values of 14,800-34,035 s^-1 M^-1 over a broad pH range^[3].
Z-Nle-Lys-Arg-AMC is an excellent substrate for purified recombinant mouse cathepsin B, exhibiting the highest specific activity and high catalytic efficiency at pH 4.6-7.2^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1135364-14-5
Molecular Weight 706.83
Formula C₃₆H₅₀N₈O₇
SMILES

CCCC[C@@H](C(N[C@H](C(N[C@@H](CCCNC(N)=N)C(NC1=CC(O2)=C(C=C1)C(C)=CC2=O)=O)=O)CCCCN)=O)NC(OCC3=CC=CC=C3)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Phan VV. Targeting Cytosolic Cathepsin B: Leveraging pH-Dependent Cleavage Preferences with Selective Inhibitors to Rescue Cell Death and Mitigate TBI-Induced Motor Deficits (Doctoral dissertation, et al.

[2]. Liolios C, et al. Prodrugs targeting prostate-specific membrane antigen against prostate cancer. Journal of Medicinal Chemistry. 2025 Jun 12;68(12):12296.

[3]. Yoon MC, et al. Distinct Cleavage Properties of Cathepsin B Compared to Cysteine Cathepsins Enable the Design and Validation of a Specific Substrate for Cathepsin B over a Broad pH Range. Biochemistry. 2023 Aug 01;62(15):2289-2300. [Content Brief]

[4]. Podvin S, et al. Activation of Cytosolic Cathepsin B Activity in the Brain by Traumatic Brain Injury and Inhibition by the Neutral pH Selective Inhibitor Probe Z-Arg-Lys-AOMK. ACS chemical neuroscience. 2025 Apr 02;16(7):1297-1308. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Z-Nle-Lys-Arg-AMC

All Cathepsin Isoforms

Biological Activity

Description

IC50 & Target

In Vitro

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Z-Nle-Lys-Arg-AMC Related Classifications

HY-155667 Related Classifications

Bioz

Keywords

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